Recovery of tissue plasminogen activator

摘要

Disclosed is a method of recovering tissue plasminogen activator from media and cell extracts. The method comprises contacting the tPA-containing solution with a silicaceous matrix material comprising covalently bound polymer having plural anionic groups. Selective elution of the tPA can produce eluants of high specific activity. The method can succeed in recovering greater than 90% of the tPA activity from the crude solution.