PREPRO TYPE ALKALI PROTEASE GENE

摘要

PURPOSE:To efficiently obtain alkali protease in a short period by culturing a fungus belonging to the genus Aspergillus. CONSTITUTION:A fungus [e.g., Aspergillus oryzae (ATCC 20386)] belonging to the genus Aspergillus is cultured to give cells, which are ground to give mRNA. The mRNA is concentrated to give alkali protease mRNA. cDNA obtained from the mRNA is integrated with vector DNA to give recombinant plasmid, which is treated with a restriction enzyme at 30-40 deg.C for 1-24 hours and the prepared solution of reaction completion is subjected to agarose gel electrophoresis to give prepro type alkali protease gene prescribed by restriction endonuclease cleavage map shown by the formula (E is EcoRI; A is Afl II; K is KpnI: X is XmnI; S is SalI; B is BgI II; B is Hind III).