METHOD FOR THE PURIFICATION OF LEUKOCYTOSIS-PROMOTING FACTOR HAEMAGGLUTININ

摘要

Described is an improved method for the purification of LPF-HA (Leukocytosis promoting factor hemagglutinin) on industrial scale which comprises contacting a LPF-HA-containing solution from culture media of Bordetella pertussis with a cellulose sulfate gel, a crosslinked polysaccharide sulfate gel, or a polysaccharide gel chemically bound to a dextran sulfate, thereby adsorbing LPF-HA on the gel, and then eluting LPF-HA from the gel. Said method can give a highly purified LPF-HA which does not contain any other proteins, lipid, saccharides, etc. and further undesirable endotoxins, and hence can be used for producing various reagents, pharmaceutical compositions and a pertussis vaccine.