发明名称 |
DIFFERENTIATION OF NUCLEIC ACID SEGMENTS ON THE BASIS OF NUCLEOTIDE DIFFERENCES |
摘要 |
CR-8694 TITLE Differentiation of Nucleic Acid Segments on the Basis of Nucleotide Differences A process for distinguishing nucleic acid segments on the basis of nucleotide differences, thereby providing a rapid, convenient means for detecting mutation, is disclosed. The present process comprises synthesizing separately complementary nucleic acid strands on each of at least two target nucleic acid templates using a nucleic acid polymerase and nucleoside triphosphate substrates, wherein at least one of the natural nucleoside triphosphate substrates is replaced with a mobility-shifting analog; denaturing the synthesized strands from the templates if necessary; and comparing the mobility of the separately synthesized strands through a size-fractionation medium. A difference in mobility indicates that the target nucleic acid templates contain different numbers of total nucleotides and/or different numbers of nucleotides complementary to the mobility-shifting analogs. Thus, the process of the present invention can be used to distinguish homologous DNAs or RNAs differing by nucleotide substitutions that affect the number of mobility-shifting analog residues incorporated into synthesized strands, as well as to distinguish differences due to nucleotide insertion or deletion. |
申请公布号 |
CA2003038(A1) |
申请公布日期 |
1990.05.15 |
申请号 |
CA19892003038 |
申请日期 |
1989.11.15 |
申请人 |
DU PONT (E. I.) DE NEMOURS AND COMPANY |
发明人 |
KORNHER, JOHN S.;LIVAK, KENNETH J. |
分类号 |
G01N33/58;C07H21/04;C12Q1/68 |
主分类号 |
G01N33/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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