| 摘要 |
PURPOSE:To readily, industrially and advantageously obtain the subject compound even from economically advantageous terrestrial starch by saccharifying a gelatinized starch with a general-purpose enzyme and then saccharifying the resultant product with a special enzyme produced by genetic recombination under specific conditions. CONSTITUTION:An aqueous solution of starch in 5-15wt.% concentration is initially thermally gelatinized and the resultant gelatinization product is then saccharified with two or more enzymes selected from beta-amylase, pullulanase and isoamylase. Maltogenic-alpha-amylase produced by integrating a plasmid having a part coding maltogenic-alpha-amylase which is a gene of Bacillus.stearothermophilus fitted therein into Bacillus.subtilis is then added thereto at a time of 1-24hr after starting the saccharification to continue the saccharification for 10-48hr from the start thereof. After completing the saccharification, pH is regulated to <=4.5 to remove water-insoluble components. Dextrin in the solution is subsequently hydrolyzed with a liquefying enzyme and then purified to afford a saccharide liquid containing 94.5wt.% maltose based on solid substances. |
