METHOD AND APPARATUS FOR ADJUSTING SPECIMEN FOR ELECTRON MICROSCOPE

摘要

PURPOSE:To enhance detection sensitivity for observing various kinds of cells and to improve observation efficiency by combining a specimen with a magnetic labeled antibody in an antigen-antibody reaction so that magnetic labeling can be performed. CONSTITUTION:The magnetic labeling of the specimen is performed by putting 1ml specimen and 10mul magnetic labeled antibody under a condition where incubation is performed at 35 deg.C for 2.5 hour. When several mul magnetically labeled specimen in a capillary 7 is dropped on a mesh 6 by compressing a cap 8 in a state where an electromagnet 1 is excited, the magnetically labeled specimen is surely guided to the center part of the mesh 6 and magnetically attracted. About one minute after the specimen is dropped, the excess specimen is absorbed with a filter paper in the state where the electromagnet 1 is still excited. Then, in the state where the electromagnet 1 is still excited, several mulnegative coloring liquid consisting of 1% phosphowolframic acid of pH 7 is dropped on the mesh 6 and the excess coloring liquid is absorbed with the filter paper after 30 seconds to be naturally dried. The observation efficiency can be improved by observing with an electron microscope thereafter.