| 摘要 |
<p>PURPOSE:To eliminate production of urea in wines or alcohols by making a brewing using Saccharomyces cerevisiae transformed by a plasmid vector for alginase gene destruction of a plasmid vector for alginase gene replacement. CONSTITUTION:A plasmid vector for alginase gene destruction or a plasmid vector for alginase gene replacement is cut with a restriction enzyme into straight chain DNA. Using this DNA, Saccharomyces cerevisiae is transformed into a transformant causing no urea formation. Using this transformant, wine or an alcohol is produced. The above transformant, due to deletion of alginase, will inhibit urea formation, thus eliminating the formation of ethyl carbamate derived from urea, leading to production of wines, etc., free from ethyl carbamate (a carcinogen).</p> |
