| 摘要 |
A process is described for the rapid detection of legionella antigens in body fluids, tissue homogenates or other legionella antigen-containing liquids with specific antibodies for the same, accompanied by the formation of a labelled antigen-antibody complex and the detection thereof by means of conventional immunoassays. It is characterized in that the immunoassay is mainly directed at the detection of an in vivo or in vitro processed antigen fraction with an apparent molecular weight of approximately 68 and/or approximately 25 kDa, determined by the SDS-PAGE method. It also describes a test kit for performing the process, which a) contains an antibody conjugate and b) a capture antibody. This permits a rapid, genus-wide detection of legionella antigens in liquids of the aforementioned type, particularly in the urine, with a high specificity, high sensitivity, whilst the process can be performed in a simple and rapid manner using readily accessible starting materials. |
