METHOD FOR IMMUNOASSAY USING PHOTOACOUSTIC SPECTROSCOPY

摘要

In this invention, antigen-antibody complex is labeled with a color material and photoacoustic properties of the label are determined by photoacoustic spectroscopy. As the color material, a fluorescent substance, a dye or colored particles can be used. The immune reaction of the labeled antigen or antibody is carried out in liquid under the condition that the solid phase is present. As the result of immune reaction, the labeled antigen-antibody complex is immobilized on the surface of the solid phase. Then, the solid phase is separated from the liquid and moved to the position for exposure to light and intermittently irradiated by the light. An acoustic signal given thereby is detected. According to the present invention, a pressure medium for photoacoustic determination is a gas. The method permits to detect antigen or antibody with high sensitivity.