METHOD FOR PREPARATION OF THIOHYDANTOINS AND FOR PROTEIN SEQUENCE ANALYSIS

摘要

A method for the preparation of amino acid thiohydantoins and for sequencing a protein or peptide comprises c-terminal degradation of a protein or peptide by i) coupling the c-terminal carboxyl group with thiocyanic acid or a thiocyanate to form a substituted thiohydantoin derivative and ii) cleaving the substituted thiohydantoin derivative with a strong inorganic base in the presence of a water-miscible organic solvent, to give a shortened protein or peptide and a c-terminal amino acid thiohydantoin which can be identified for sequencing purposes.