METHOD FOR THE DETECTION OF MULTIPLE DRUG RESISTANT TUMOR CELLS AND VERAPAMIL PROBES USEFUL THEREIN

摘要

A method for the detection of multiple drug resistant tumor cells is described which comprises the incubation of a sample of mammalian tumor cellular material with an effective binding amount of a labelled verapamil derivative to form a complex with any P-gp that is present, and then determining the amount of P-gp present by quantifying the amount of labelled verapamil derivative bound in the complex. Photoaffinity, radiolabelled and chemoaffinity derivatives of verapamil and the syntheses of such derivatives are described, as are a method of treatment for multiple drug resistant tumors and a diagnostic kit.