摘要 |
PURPOSE:To simply obtain the title amine and its derivative in high optical purity by subjecting a specific mixture to microorganisms capable of asymmetrical hydrolysis of the amide linkage in said mixture. CONSTITUTION:Microorganisms belonging to Rhodococcus sp. is put to culture at pH3.9-9.5 and 20-45 deg.C for 1-5 days under a desired O2 concentration in a medium containing e.g., glucose to obtain a cultured product. This product is then added to 0.1-10wt.% solution of an enantiomer mixture of an acyl derivative of amine of formula I (X1-2 are each H, nitro, halogen, OH or alkyl; COR is acyl; n is 1-3) (e.g., N-acetyl-2-amino-4-phenylbutane) followed by reaction on agitation at pH3-9 and 10-60 deg.C for 1-120hr to obtain a reaction solution. Thence, this reaction solution is extracted and purified, thus separately obtaining the objective optically active (S) form of an amine of formula II (e.g., 2-amino-4- phenylbutane) and optically active (R) from of an amine acyl derivative of formula left. |