A test to diagnose the presence of Johne's disease is disclosed. In one aspect it involves using a DNA segment that will hybridize to M. paratuberculosis but not to other bacteria typically found in bovine feces. Another aspect of the invention is providing a process for breaking the cell wall of mycobacteria using dense beads in the presence of phenol.
申请公布号
US4918178(A)
申请公布日期
1990.04.17
申请号
US19870112179
申请日期
1987.10.22
申请人
WISCONSIN ALUMNI RESEARCH FOUNDATION
发明人
HURLEY, SARAH S.;SPLITTER, GARY A.;WELCH, RODNEY A.