| 摘要 |
<p>This invention relates to the use of functional reporter molecules in the detection and measurement of nucleic acid sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of an adduct having an oligonucleotide probe sequence linked to 1) a sequence capable of initiating primer extension or transcription, and 2) a complement of 1). The adduct assumes a hairpin structure rendering the sequence of 1) inactive. Upon hybridization of the oligonucleotide probe sequence with target nucleic acid sequence, the adduct is target-activated and sequence 1) is available for processing to prove detection and measurement of the target nucleic acid sequence.</p> |
