发明名称 |
Purification and refolding of recombinant proteins. |
摘要 |
<p>The present invention discloses a new method for solubilizing and refolding recombinant proteins expressed as granules. The method involves sulfitolysis and the formation of a precipitate of protein-S-sulfonate by warming. The precipitate has been found to contain protein in high purity. In addition, proper folding takes place if the desired protein is fully reduced and passed through an intermediate concentration of denaturant which allows for a transition between its folded and unfolded states.</p> |
申请公布号 |
EP0361830(A2) |
申请公布日期 |
1990.04.04 |
申请号 |
EP19890309726 |
申请日期 |
1989.09.25 |
申请人 |
ELI LILLY AND COMPANY |
发明人 |
BOBBITT, JESSE LEROY;MANETTA, JOSEPH VINCENT |
分类号 |
C12P21/00;C07K1/113;C07K1/30;C07K14/00;C07K14/52;C07K14/54;C07K14/55;C07K14/555;C07K14/575;C07K14/61;C07K14/65;C12P21/02 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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