| 摘要 |
<p>New probes are provided for the detection of target RNA comprising RNA templates replicated by Q-beta replicase having a target specific probe attached through specific sequences at both the template's 3 min and 5 min ends whereby hybridization of the probe with target results in the formation of a cleavage inducible ribozyme. Following cleavage, the RNA template may be advantageously amplified by Q-beta replicase resulting in the generation of a large signal correlatable to the presence of RNA target in the sample. Suitable methods employing such probes are also provided.</p> |
