Alpha factor leader sequence directed secretion of insulin

摘要

Methods and compositions are provided for efficient production of polypeptides having insulin activity. The proinsulin gene is joined to secretion and processing signals for expession and secretion in yeast. The product may be obtained in enhanced yield in the nutrient medium. Cell line AB103(PYinsl) was deposited at the A.T.C.C. on Apr. 6, 1983 and given Accession No. 20,670.