POTATO TUBER FORMING AND INDUCING AGENT AND METHOD FOR FORMING AND INDUCING POTATO TUBER

摘要

PURPOSE:To surely form and induce large amounts of potato tuber by adding ascorbic acid and jasmonic acid compounds to a culture medium. CONSTITUTION:A stem fragment containing a terminal bud or nod reared by shoot tip culture or rooting transfer method of potato plant is reared in tissue culture medium (e.g. Linsmaier & Skoog) for about 4 weeks to provide an aseptic shoot. 10-5000ppm ascorbic acid and 0.3-12ppm jasmonic acid compound expressed by formula I or formula II (R1 and R2 are H or 1-10C alkyl; R2 is H, OH, O-D-glucopyranose) and as necessary 0.5-10ppm cytokinins compound (e.g., kinetin) used as a potato tuber-forming and inducing agent are added to a culture medium containing the above-mentioned aseptic shoot and the shoot is cultured for 2-4 weeks to form potato tuber at the nod of aseptic shoot.