| 摘要 |
PURPOSE:To obtain a novel lipase having optimum pH on the high alkali side and being low in optimum temperature and suitable for detergent by culturing a yeast belonging to the genus Candida and having lipase-producing ability in a culture medium. CONSTITUTION:A yeast (e.g., SD-701 strain, FERM P-10137) belonging to the genus Candida and having a lipase-producing ability is cultured in a culture medium to produce a lipase and collect the lipase from the cultured product. In the obtained novel lipase, optimum pH when triolein emulsion is used as a substrate is 9-10 and optimum temperature when the same substrate is used is 37-40 deg.C and molecular weight measured by SDS-polyacrylamide electrophoresis method is 35000+ or -20000 and molecular weight measured by gel permeation method is 43000. Isoelectric point measured by isoelectric polyacrylamide gel electrophoresis is 4.8+ or -0.2. |
