| 摘要 |
PURPOSE:To prepare an antitumor substance by using a specific T-23 substance-producing strain. CONSTITUTION:Streptomyces T-23 strain (FERM-P No.6141) belonging to Streptomyces rishiriensis, is cultured in a conventional medium for the cultivation of actinomycetes, by submerged technique, at about 20-35 deg.C for about 17-96hr, and the active fraction is extracted mainly from the bacterial cells using a solvent (e.g. mixture of acetone and water). The extract is concentrated, and mixed with petroleum ether, hexane, etc. The resultant precipitate is purified by chromatography to obtain T-23-I substance (the compound of formulaI) and T- 23-II substance (the compound of formula II). |
