摘要 |
PURPOSE:To effectively obtain a large amount of natural tocopherol regardless of seasons without requirement of a vast field for cultivation by tissue culture of a Bryophyta plant and isolation of tocopherol from the cultured material. CONSTITUTION:A Bryophyta plant, preferably such as Marchantia polymorpha L. or Jungermannia amakawana Grolle is inoculated on a synthetic nutrient culture medium and subjected to tissue culture. The resultant cultured material is then subjected to extraction using a solvent such as n-hexane and purification by adsorption chromatography, etc., thus obtaining the objective tocopherol. |