| 摘要 |
Zones of ampholytic substances which have been isoelectrically focused in a separation medium are mobilized for analytical or preparative purposes by either introducing anions other than hydroxyl ions to the separation medium toward the catholyte end or adding cations other than protons to the separation medium toward the anolyte end to produce a pH shift in the separation medium, thus restoring a charge to the focused ampholytes so that they will mobilize under the influence of a voltage applied across the medium. In preferred embodiments, the anion or cation introduction is achieved by supplementing the catholyte or anolyte, respectfully, with a strong electrolyte and permitting the cations or anions to migrate into the separation medium under the influence of the electric field. Detection is achieved by permitting the focused zones to migrate past a fixed point in the separation medium. Alternatively, detection of the zones outside of the separation medium and/or recovery of the individual proteins themselves may be achieved in the same way provided the receiving electrolyte has an appropriate pH which will permit migration of the zones to continue into the electrolyte itself.
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