发明名称 Alginate beads containing living cells and process for producing them
摘要 The process consists in preparing 10, 12, 14 a suspension of sodium alginate in a culture medium suitable for culturing alveolar macrophages; in preparing 16, 18, 20, 22 a suspension of alveolar macrophages AM, having previously phagocytised respirable particles, in a solution containing the culture medium and foetal calf serum; in mixing 24 the alginate suspension and cellular suspension; in extruding 26 the mixture in order to form droplets; in gelling 28 these droplets in a gelling medium containing culture medium, foetal calf serum and calcium chloride and then in dispersing the alginate beads obtained in a culture medium supplemented with foetal calf serum and calcium chloride. The alginate beads obtained are used to study the dissolution of particles by the alveolar macrophages. <IMAGE>
申请公布号 FR2636645(A1) 申请公布日期 1990.03.23
申请号 FR19880012100 申请日期 1988.09.16
申请人 COMMISSARIAT A ENERGIE ATOMIQUE 发明人 DANIEL NOLIBE;PIERRE-NOEL LIRSAC;HENRY METIVIER
分类号 C12N11/04;G01N33/50 主分类号 C12N11/04
代理机构 代理人
主权项
地址