摘要 |
Protein derivatives of formula (1) A-CO-N(R<1>)N(R<2>)-Z<1>- @-Z<2>-N(R<3>)NHR<4> (1) wherein A-CO is the residue of a protein or peptide A-CO2H (especially where -CO2H is the C-terminal carboxyl group of the protein or peptide); R<1>, R<2>, R<3> and R<4>, which may be the same or different, and which may be present or absent, is each a spacer group, X is an oxygen or sulphur atom; and the salts thereof. The compounds may be prepared by a condensation reaction, especially in the presence of an enzyme capable of catalysing the formation of a peptide bond. The compounds may be used to form protein conjugates in which a group of interest such as another protein or peptide or an effector or reporter group is coupled to the protein through a -N(R<1>)N(R<2>)-Z<1>-C(=X)-Z<2> -N(R<3>)N= group. |