| 摘要 |
Modified nucleotides are provided which have the structure <IMAGE> wherein R1 is a reactive group derivatizabale with a detectable label, R2 is an optional linking moiety including an amide, thioether or disulfide linkage or a combination thereof. R3 is hydrogen, methyl, bromine, fluorine or iodine, R4 is hydrogen, an acid-sensitive, base-stable blocking group or an acyl capping group, R5 is hydrogen or a phosphorus derivative, R6 is H, OH, or OR where R is a protecting group and x is an integer in the range of 1 and 8 inclusive. Methods of synthesizing the derivatizable nucleotide are disclosed, as are labeled polynucleotide probes prepared therefrom.
|
