Derivatization of amines for electrochemical detection
摘要
Primary and secondary amines in biological fluids are selectively derivitized by reaction with esters of N-hydroxysuccinimide or N-hydroxysulfosuccinimide to form an N-acylated derivative. Those N-acylated derivatives may then be extracted into a polar organic phase and extracted. Non-electroactive amines may be made suitable for coulometric analysis by selecting the ester used so as to attach an electroactive group to the amine. For example, histamine may be reacted with the Bolton-Hunter reagent to form a suitable derivative in high yield.
申请公布号
US4908322(A)
申请公布日期
1990.03.13
申请号
US19880290279
申请日期
1988.12.27
申请人
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF HEALTH AND HUMAN SERVICES
发明人
JACOBSON, KENNETH A.;KIRK, KENNETH L.;LINNOILA, MARKKU I.;MILLER, THOMAS;MINE, KAZUNORI