| 摘要 |
PURPOSE:To provide an expression factor containing specific replicator, promoter region, restriction enzyme nicking side and terminator region and capable of expressing an exogenote in a cell of Candida maltosa which is an anascosporogenous yeast. CONSTITUTION:The objective expression vector contains (1) a replicator functioning in the cell of Candida maltosa, (2) a promoter region originated from the chromosome DNA of Candida maltosa, (3) restriction enzyme nicking site at the downstream of the promoter region and (4) a terminator region originated from LEV2 gene of Saccharomyces cerevisiae. The nucleotide sequence of the DNA containing the promoter region is preferably the one expressed by the formula. |
