| 摘要 |
An extraction method of edible red pigment from cockscomb (Celosia cristata) is presented. Thus, cockscomb is dried at cold and dark place, crushed by mortar, and preserved at -70oC in a sealed bottle. The material (20g) is treated with 500-1000 ml of a solvent containing water, ethanol, methanol, and 0.1-5% of HCl to extract the pigment of cockscomb. The pigment is loaded on a preparative agarose gel electrophoresis (0.1M Na-citrate buffer, ph 4.5; 5mA/cm; 5 hrs). After the electrophoresis, the red pigment portion is cut, eluted with distilled water, and centrifuged at 10,000rpm for 30 min to give a pure red pigment.
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