ON-LINE AUTOMATIC ANALYZING INSTRUMENT FOR BIOREACTOR

摘要

PURPOSE:To attain continuation and automation of a series of pretreating processes by assembling a filter which filters pH-adjusted samples in a turntable. CONSTITUTION:The sample of the product of a bioreactor 8 is sucked into and held in a sample loop 7 through a low-pressure six-way changeover valve 6 while the gas produced from the sample is exhausted by means of an exhaust pump 22 by opening a solenoid valve 5. A fixed quantity of a buffer liquid for dilution having a desired pH value in a tank 1 is fed to a dilution tank 9 by pushing out the sample into a loop by means of a pump 3 through a changeover solenoid valve 4 and the valve 6 after the buffer liquid is passed through a filter 2 and mixed with the sample by means of a stirrer. Thus the sample is diluted and adjusted in pH. The sample liquid thus adjusted is fed to a filter 32 by means of a pump 14 through a solenoid valve 23 and small holes 36 and 33 through pipelines and filtered by the filter under a pressure. The liquid filtered by the filter 32 is fed to a sample loop 17 through small holes 31 and 27 and a hexagonal valve 16 and further fed to a column 18. A solvent for gradient in a reservoir tank 20 is sent to the column 18 by means of an HPLC pump 19.