Procaryotic carbonyl hydrolases and mutants thereof, and methods for producing and using them.

摘要

DNA sequences are disclosed encoding <Underline style="single">B. amyloliquefaciens</Underline> and <Underline style="single">B. subtilis</Underline> subtilisins and <Underline style="single">B. subtilis</Underline> neutral protease. Using DNA derived from these sequences as hybridisation probes, genes for these and other closely related enzymes can be cloned from procaryotic sources, identified by their carbonyl hydrolytic acitivity and expressed in a suitable recombinant host. Moreover, the DNA can be mutated to produce enzymes with altered characteristics which can be screened for to identify desired changes, and thereafter the modified enzymes obtained by expression of the muted genes in suitable recombinant hosts.