IDENTIFICATION OF ALLELE SPECIFIC NUCLEIC ACID SEQUENCES BY HYBRIDIZATION WITH CROSSLINKABLE OLIGONUCLEOTIDE PROBES

摘要

Hybridization method for discriminating between complementarity and partial complementarity of DNA base sequences. Kinetic covalent entrapment of PCR-amplified target DNA is achieved through the use of crosslinkable probes. A crosslinking site is introduced into the target DNA via the PCR amplification process. Problems with renaturation of target DNA in the PCR process and in the hybridization reaction are minimized.