发明名称 |
Cascade immunoassay by multiple binding reactions |
摘要 |
A method for enzyme immunoassay includes contacting under binding conditions a liquid suspected of containing an analyte, an antianalyte affixed to a solid support and a tracer having an enzyme conjugated thereto. A bound fraction is separated from the liquid and incubated in a second liquid with a masked ligand. The masked ligand is converted by the enzyme on the bound fraction to give free lignad which binds to an antiligand. A signal system, such as a signal enzyme and substrate therefor, or a label-loaded vesicle and vesicle lysing agent, is added to generate a signal used to detect or measure the analyte in the liquid. The invention includes a kit of materials useful in performing the assay of the invention.
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申请公布号 |
US4904583(A) |
申请公布日期 |
1990.02.27 |
申请号 |
US19870053896 |
申请日期 |
1987.05.26 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
MAPES, JAMES P.;HOKE, RANDAL A. |
分类号 |
G01N33/543;G01N33/58 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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