摘要 |
<p>A method of carrying out an assay for an enzyme, for example as part of an enzyme immunoassay, comprises reacting the enzyme with a development reagent in the presence of a chelating agent such as EDTA, which is a progressive enzyme inhibitor, under conditions such that the ability of the enzyme to react with the development reagent to produce the determinable change is progressively inhibited as the reaction progresses.</p> |