IDENTIFICATION OF ALLELE SPECIFIC NUCLEIC ACID SEQUENCES BY HYBRIDIZATION WITH CROSSLINKABLE OLIGONUCLEOTIDE PROBES
摘要
Hybridization method for discriminating between complementarity and partial complementarity of DNA base sequences. Kinetic covalent entrapment of PCR-amplified target DNA is achieved through the use of crosslinkable probes. A crosslinking site is introduced into the target DNA via the PCR amplification process. Problems with renaturation of target DNA in the PCR process and in the hybridization reaction are minimized.
申请公布号
WO9001563(A1)
申请公布日期
1990.02.22
申请号
WO1989US03189
申请日期
1989.07.24
申请人
CIMINO, GEORGE, D.;HEARST, JOHN, E.;ISAACS, STEVEN, T.;LEVENSON, COREY;SAIKI, RANDALL, K.
发明人
CIMINO, GEORGE, D.;HEARST, JOHN, E.;ISAACS, STEVEN, T.;LEVENSON, COREY;SAIKI, RANDALL, K.