| 摘要 |
<p>Tissue produced by culturing isolated epithelial cells (preferably squamous cells) will retain the ability to express the phenotypic characteristics of the isolated cells when the tissue is transplanted into a new region of body surface. Thus defects in the epithelium (e.g., burns, ulcers and congenital abnormalities) can be treated by obtaining a sample of living epidermal cells from a region of the patient's epithelium (e.g., sole of the foot, palm of the hand or scalp) having a site-specific phenotypic characteristic (e.g., a thick stratum corneum or a stratum lucidum positioned between the stratum granulosum and the stratum corneum) which is desired for the graft at the defective region. These epidermal cells are cultured, according to methods well-known to those skilled in the art, to produce transplantable living cells, e.g. an aggregation or sheet of living tissue. This tissue is then transplanted to the site of the defect where it expresses the phenotypic characteristic in a region of the patient's epithelium that may or may not ordinarily manifest this characteristic.</p> |
