| 摘要 |
A method for temporarily increasing the hydrophilic properties of a material, such as a microbial antigen or antibody thereto, comprises contacting the material with a detergent compound containing a modifiable group and subsequently modifying said group (e.g. by cleavage, oxidation or reduction) by addition of a suitable reagent so as to decrease or destroy the detergent properties of said compound. The method may be used to prepare an analyte for assay, subsequent removal of the detergent being made more efficient or even eliminated by means of the modifying reagent. Detergent compounds of general formula CH3(CH2)n-A- (CH2)mOW (or their salts) where A is -S- or -S-CH=CH-; W is H or SO3H; and n is 2-4 and m is 6-11 when A is -S- or n and m are each 1-10 when A is -S-CH=CH- are also claimed. |
