| 摘要 |
PCT No. PCT/FR86/00378 Sec. 371 Date Jul. 6, 1987 Sec. 102(e) Date Jul. 6, 1987 PCT Filed Nov. 7, 1986 PCT Pub. No. WO87/02879 PCT Pub. Date May 21, 1987.The invention relates to a container for fertilization of human ovocytes in the absence of CO2 which may be done in an incubuator at 37 DEG C. or in the vaginal cavity. The container of cylindrical shape has smooth and rounded walls 1 whose thickness is 2 mm in the body 2 of the cylinder but only 1 mm at the neck 3. On the outer wall of the neck is seen a screwthread 4 permitting external screwing on of the plug 12 whose sides are smooth and rounded. The blind end 5 of the container is rounded, the other 6 is flat and has a round orifice 7, which orifice is closed by a fine membrane 9 permitting hermeticity of the tube in order to avoid disorders of the culture medium 8 present in the container as of manufacture. Above the liquid remains about 200 mu l of gas 10. The inner walls 11 are smooth and rounded. After depositing the ovocytes and spermatozoa necessary for fertilization, and therefore after rupture of the membrane, hermeticity will be effected by two seals, after screwing on the plug, a circular one 13 at the neck of the tube, the other 14 on the endwall of the plug which will be flattened against the orifice of the tube 7 by a relief portion, located on the endwall of the plug 15, whose diameter is substantially that of the orifice. The container will be held in the posterior fornix of the vaginal cavity by a flexible ring comprised of a metal strip 17 sheathed in rubber 18 to which is fixed a rubber pouch 19 in which the container is placed. This device thus permits inter-vaginal, fertilization of human ovocytes in the absence of CO2-enriched air, the vaginal cavity replacing the incubator conventionally used.
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