Method for the purification of a biologically active agent

摘要

Method for the purification of a biologically active agent. The method is characterized by comprising: shaking the cystic fluid of female breasts, type 1, with a first polar solvent, and discarding the precipitate; mixing the dissolved factor with a second polar solvent, and recovering an organic phase which contains said factor; said second polar solvent being less polar than the first solvent; separating the second polar solvent from said organic phase; freeze-drying the aqueous phase; extracting the freeze-dried product with a methanol-water mixture; extracting the freeze-dried product with a methanol-water mixture; subjecting the extracted freeze- dried product to hydrophobic affinity chromatography; and recovering the fractions which contain said factor. <IMAGE>