PRODUCTION OF MONOCLONAL ANTIBODY, ANTIBODY-PRODUCING HYBRIDOMA AND ANTIBODY

摘要

PURPOSE:To produce a versatile antibody specific to T cell-type tumor by producing a monoclonal anti-human T cell receptor idiotype antibody using a specific hybridoma. CONSTITUTION:An antibody-producing cell collected from the spleen of a mouse immunized with a human T cell-type leukemia cell strain is fused with a mouse myeloma cell to obtain an antibody-producing hybridoma. The obtained hybridoma is selectively grown on a HAT-containing medium and screened to obtain a hybridoma capable of producing human T cell receptor idiotype monoclonal antibody (anti-hTcR-MoAb). The hydridoma is cultured in a liquid medium or in the abdominal cavity of a thymectomized nude mouse. The antibody-containing liquid is centrifuged and the MoAb is separated from the supernatant liquid and purified. The obtained MoAb is suitable as a biopharmaceutical which can be administered to human as a remedy or missile therapeutic agent.