| 摘要 |
<p>Method for the automatic registration of images of seriated cross-sections studied and observed with optical and electronic transmission microscopes. The invention relates to a method for automatically matching seriated cross-sections observed successively with microscopes. Prior to the cutting with the microtome or with an ultramicrotome, three or four regular holes, as fine as possible, are drilled either mechanically or with a pulse laser into the block containing the preparation to be studied. The preparation is cut into seriated regular cuttings in the order of 20 to 60 nanometres for the electronic microscopy and in the order of the micron for the optical microscopy. The images obtained with the microscope are input, digitized and stored in a data processing system. They are normalized by data processing particularly as to the contrast enhancement and the grey level stabilization. The images are then registered by using a reverse deformation function previously calculated from marks embodied by the marking holes. The method according to the invention is particularly intended for reconstituting the tridimensional information from planar seriated cuttings visualized either by means of optical microscopes or by means of electronic transmission microscopes. The method according to the invention allows, by defining a fixed mark, to automate the research of a zone of one cross-section on the other for seriated cross-sections observed either with an electronic transmission microscope or with an optical microscope of which the motions of the optic carrier grids and the control parameters are calculated by a computer.</p> |
