SENSOR WITH IMMUNOCHEMICAL MEMBRANE CHEMICALLY BOUND TO A SEMICONDUCTOR DEVICE

摘要

A sensor and its methods of preparation are described, the sensor containing an immunochemical membrane and an EOS or CHEMFET device containing on its surface silicon oxide adhering to said membrane by means of a polysiloxane matrix, characterised in that the immunochemical membrane is formed from a polymeric multilayer consisting of an antibody or protein A, said antibody or protein A being polymerized together with support biopolymers and said immunochemical membrane being chemically bound to the polysiloxane matrix by bifunctional coupling agents, the polysiloxane matrix being chosen from functional organosilanes of general formula: where R, R, R, which can be identical or different, are alkyl or alkoxy groups containing between 1 and 10 carbon atoms; R = (CH2)m X (CH2)n where X is CH2 or a mono or polycondensed aromatic group or NH or O, and m and n, which can be equal or different, are whole numbers between 0 and 10, but not 0 if X is NH or O; Y is -NH2 or -OH or -SH; or from functional organosilanes of general formula: where R1, R2, which can be identical or different, are Cl, Br, CH3, NO2, NH2 or H; R, R, R, which can be identical or different, are alkyl or alkoxy groups containing between 1 and 10 carbon atoms; R can be an alkyl, aminoalkyl, aminoalkylaryl or alkylaryl group containing between 1 and 10 carbon atoms.