Performing an enzyme-catalysed reaction.

摘要

A process for performing an enzyme-catalysed reaction in a reaction mixture comprising two phases, a first aqueous phase containing an enzyme which is not accessible and a second substantially non-aqueous phase containing a non-water miscible solvent wherein a compound derived from fluorescein or a substituted fluorescein is present for the purpose of measuring aqueous pH by monitoring spectroscopic properties. Novel compounds derived from fluorescein or a substituted fluorescein which may be used in the process and a method for producing some of the novel compounds are also claimed.