PRODUCTION OF XBAI RESTRICTION ENDONUCLEASE AND METHYLASE

摘要

The present invention is directed to a method for cloning and producing the XbaI restriction endonuclease by 1) introducing the restriction endonuclease gene from Xanthomonas badrii into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the XbaI restriction endonuclease, and 3) purifying the XbaI restriction endonuclease from the fermented host which contains the vector encoding and expressing the XbaI restriction endonuclease activity.