PREPARATION OF LIPID CONTAINING UNSATURATED FATTY ACID

摘要

PURPOSE:To provide the subject lipid having an improved unsaturated fatty acid content by subjecting a lipid-producing fungus to a feed culture under a condition restricting the supply of a carbon source or to a substitution culture under a condition free of the carbon source. CONSTITUTION:An unsaturated fatty acid-producing fungus such as Mortierella.alpina is inoculated in a medium containing a saccharide such as glucose or fructose as a carbon source in an amount of the most suitable saccharide concentration or less for the growth of the lipid-producing fungus to begin the culture of the fungus. the saccharide is subsequently added to the medium in an amount of the most suitable saccharide concentration or less for the growth to multiply the fungus. The objective lipid is obtained by continuing the culture in a state free of the carbon source in the multiplication stationary period of the lipid-producing fungus or near the period after the multiplication and/or the culture of the fungus is begun.