发明名称 Method and apparatus for separating mononuclear cells from blood.
摘要 <p>A blood separation device includes a collection tube (2) containing a first layer (10) of a thixotropic gel-like substance and a second layer (12) of a newtonian gel-like substance which is positioned below the first layer (10). The first layer (10) of the thixotropic substance is positioned between the second layer (12) of the newtonian substance and a blood sample received by the tube (2), prior to centrifuging the tube, and acts as a barrier between the second layer (12) and the blood sample to prevent intermixing of the second layer and the blood sample. The thixotropic gel-like substance of the first layer (10) has a specific gravity which is greater than the newtonian gel-like substance of the second layer (12) whereupon centrifugation of the tube (2), the first layer (10) moves to a position below the second layer (12). The second layer (12) of the newtonian gel-like substance acts as a density separation medium, the newtonian gel-like substance being formed with a specific gravity such that the second layer (12) is adapted to assume a position in the collection tube (2), upon centrifugation of the tube (2), between the mononuclear cells (22) and the heavier components (18,20) of the blood sample placed in the tube (2).</p>
申请公布号 EP0350851(A1) 申请公布日期 1990.01.17
申请号 EP19890112624 申请日期 1989.07.11
申请人 BECTON DICKINSON AND COMPANY 发明人 SMITH, WARD C.
分类号 G01N33/48;A61B10/02;B01D21/26;B01L3/14;G01N33/49;G01N33/50 主分类号 G01N33/48
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