FREE FLOW TYPE ELECTROPHORETIC APPARATUS

摘要

PURPOSE:To balance the inflow and outflow of ions between electrode chambers and electrophoretic space and to suppress the rise of the electrical conductivity of a buffer soln. by spreading an electrode membrane obtd. by vertically joining an upper cation membrane and a lower anion membrane at the anode side of a free flow type electrophoretic apparatus and an electrode membrane obtd. by vertically joining an upper anion membrane and a lower cation membrane at the cathode side. CONSTITUTION:An electrode membrane 11 obtd. by vertically joining an upper cation permeable membrane (cation membrane) 11a and a lower anion permeable membrane (anion membrane) 11b of the same size is spread at the anode side of an electrophoretic apparatus. An electrode membrane 12 obtd. by vertically joining an upper anion membrane 12b and a lower cation membrane 12a of the same size is spread at the cathode side to form an electrophoretic chamber and electrode chambers. When voltage is impressed between both electrodes 4, 4, cations from the anode and anions from the cathode enter the electrophoretic chamber through the membranes 11, 12 in the upper part. In the lower part, anions enter the electrode chamber adjacent to the anode and cations enter the electrode chamber adjacent to the cathode because ion exchange membranes having opposite polarities are present and the concn. of ions in the electrophoretic chamber is kept nearly constant, so any electric current affects the concn. of ions in the electrophoretic chamber.