| 摘要 |
<p>PURPOSE:To enhance berberine content in a cultured tissue and efficiently mass-produce berberine by adding a culture solution of bacteria after culturing sterilized bacteria into a culture solution and culturing calli of a plant belonging to the genus Coptis. CONSTITUTION:Subcultured calli or cultured tissues are inoculated into a liquid culture medium. A culture solution of bacteria after culturing sterilized bacteria, together with, e.g., a carbon source (e.g., sucrose) in 1-20g/l concentration and a nitrogen source (e.g., casamino acid) in 0.1-2g/l concentration, is added to the above-mentioned liquid culture medium after the logarithmic growth phase or in the initial stationary phase in the propagation cycle of the afore-mentioned calli to culture the above-mentioned calli.</p> |
