NOVEL STIMULATION FACTOR FOR HUMAN GRANULOCYTE MACROPHAGE COLONY

摘要

PURPOSE:To provide a novel polypeptide exhibiting the activity comparable to that of known stimulation factor and having an amino acid sequence of a stimulation factor for human granulocyte macrophage colony provided that the 1st and the 2nd amino acid residues are depleted. CONSTITUTION:The objective polypeptide has an amino acid sequence corresponding to the amino acid sequence of a stimulation factor for human granulocyte macrophage (GM-CSF) shown in the figure provided that the 1st (Met) and the 2nd (Ala) amino acid residues are depleted and that the sequence is composed of the amino acid sequence of from the 3rd to the 128th residues. The polypeptide can be prepared by culturing a transformed E.coli and separating and recovering the stimulation factor for human granulocyte macrophage produced in the microbial cell from the cell by reversed phase high-performance liquid chromatography to separate the objective factor from conventional factor.