| 摘要 |
PURPOSE:To directly introduce a desired DNA into the cell of lactobacillus by adding an extraneous DNA to a suspension produced by suspending lactobacilli in a buffer solution and applying pulse electric current to the mixture. CONSTITUTION:Lactobacillus cells (e.g., lactobacillus casei) of the prophase of logarithmic growth phase are suspended in a buffer solution (e.g., sodium phosphate buffer solution) of pH 5.5-9 and mixed with >=0.01mug/cuvette of extraneous DNA (e.g., plasmid pAMbetaIN 0.1) and, as necessary, <=30wt.% of polyethylene glycol. The suspension is introduced into a vessel having a pair of plate electrodes and a high-voltage pulse current is passed through the suspension at 0-40 deg.C to effect the electroporation. The objective transformant is selected from the treated product by conventional selection process. |
