发明名称 |
SEQUENCING DNA; A MODIFICATION OF THE POLYMERASE CHAIN REACTION |
摘要 |
The invention provides a process wherein a biotinylated oligonucleotide primer and an oligonucleotide primer which has not undergone biotinylation are used when amplifying a DNA sequence to facilitate separation of the DNA strands following the polymerase chain reaction process. The biotinylation/PCR product is then exposed to a support which will selectively bind the biotinylated strand to allow selective elution of the product. |
申请公布号 |
AU3835089(A) |
申请公布日期 |
1990.01.05 |
申请号 |
AU19890038350 |
申请日期 |
1989.06.01 |
申请人 |
THE UNITED STATES OF AMERICA, REPRESENTED BY THE SECRETARY, UNI |
发明人 |
LLOYD G. MITCHELL;CARL R. MERRIL |
分类号 |
C12N15/09;C07H21/00;C07H21/04;C12N15/10;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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